BRILLIANT GREEN BILE 2% BROTH 500g

Specifications

Description

This medium was formulated by Durham and Schoenlein² to selectively recover organisms of the coli-aerogenes group. The bile and brilliant green components inhibit the Gram-positive organisms, whilst the coli-aerogenes group are recognised by the rapid formation of gas during lactose fermentation³. It is important that the inhibitory agents in the medium are balanced with the nutrient and mineral components, so that Clostridium and Bacillus spores do not give false positive reactions in the medium i.e. gas formation. Brilliant Green Bile Broth is used in water, dairy and food analysis^4,5,6,7,8.

The addition of 4-methylumbelliferyl-β-D-glucuronide (MUG) (BR0071) to this medium will enhance the detection of Escherichia coli. See MUG Reagent (BR0071) under Biological Reagents for further details.