LAURYL TRYPTOSE BROTH 500g

Description
Lauryl Tryptose Broth provides a selective medium which is used for the detection of coliform organisms in water, dairy products and other foods. The APHA¹ recommend that Lauryl Tryptose Broth should be used for the Most Probable Number (MPN) presumptive test of coliforms in waters, effluent or sewage as a confirmatory test of lactose fermentation with gas production for milk samples ( APHA² ) and for the detection of coliforms in foods ( APHA³ ).

Surface active agents have long been used as the inhibitory ingredients in selective media. MacConkey⁴ introduced bile salts for this purpose and later Albus and Holm³ working with lactobacilli found that sodium ricinoleate exerted a selective action. The development of synthetic wetting agents opened up new fields of investigation. Mallmann and Darby⁶ after comparative tests with a large number of these compounds, showed that sodium lauryl sulphate gave the best results in selective media for the coliform group.

Lauryl Tryptose Broth was designed to promote a rich growth and copious gas production from small inocula of coliform organisms. Aerobic sporing bacteria are completely inhibited. The advantage in using this product is that in addition to giving the fermentation reaction typical of MacConkey Broth it can also be directly tested for the presence of indole. Unlike MacConkey Broth, the medium contains no indicator, but this can be added (if required) after incubation.

Specification