Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity, and provides excellent orthogonality to low pH reversed-phase LC-MS gradients. The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a high-pH fractionation protocol.